MORPHOLOGICAL CHARACTERIZATION AND MOLECULAR IDENTIFICATION OF S. MELONGENA L. AND D. STRAMONIUM L

Abstract

Two important plant Solanum melongena L. and Datura stramonium L. belonging to family Solanacae were taken due to medicinal and genetical significance purpose. Eggplant (S. melongena L.) is an important crop in terms of economic and genetic significance in tropical and subtropical regions. Eggplant has antioxidants like vitamins A and C, which help protect your cells against damage. It's also high in natural plant chemicals called polyphenols, which may help cells do a better job of processing sugar if you have diabetes. The parts of plant D. stramonium L. are utilized as a part of people’s medication for their anti-asthmatic, anti-spasmodic, and anti- parkinsonian properties. D. stramonium has been utilized broadly in medication. The juice of its fruit is applied to the scalp, to treat dandruff and falling hair. An accurate identification of plants is essential to the safety, efficacy, and effectiveness of herbal remedies. By using DNA barcoding, we identified two locally grown plants. To elucidate the variation at molecular level this study was done by DNA barcoding. An analysis of DNA barcoding was conducted to elucidate the variation at the molecular level. With DNA barcoding, species can be identified quickly and accurately by using short, standardized gene regions. As part of our present research, DNA was extracted, gel electrophoresed, PCR amplification, DNA sequenced, and analyzed. Using conventional PCR techniques, genomic DNA was extracted from plant leaves samples and amplified. Plant species were compared and differentiated using short sequence diversity in matK and rbcL genes of plastid genomes. Purified and sequenced samples of both plants were then obtained. Based on NCBI database blasting, all samples show high similarity to homologs.