Abstract
Objective: To evaluate the association between Variations in Hematological Parameters (Platelets count, leucocyte count), Dengue serological markers (NS1, IgG, IgM) and Patient demographics (Age and Gender) in patients presenting with dengue fever. Methods: A cross-sectional comparative study was conducted across healthcare facilities in Mardan, Pakistan. Data from 250 serologically confirmed dengue patients were analyzed. Complete blood counts and rapid immunochromatographic serological results were correlated with demographic data using ANOVA and Chi-square analyses. Results: The cohort (M = 30.56 years; 67.6% male) predominantly presented with NS-1 only positivity (n=143), Patients with combined IgG and IgM positivity exhibited significantly higher WBC counts compared to NS1 only patients (p = 0.010). Age demonstrated a significant inverse correlation with both platelet (p < 0.001) and WBC counts (p < 0.001), with pediatric patients presetting the highest baseline levels. Female patients exhibited significantly higher platelet counts than males (p = 0.010). Conclusion: Hematological parameters in dengue infection are significantly modified by the patient’s immunological stage and demographic profile. In resource-constrained settings, combining qualitative serological profiles with CBC data provides a necessary-based context for clinical interpretation.