Abstract
Artificial sweeteners like sodium saccharine and other related products have widely been used as sweeteners in different kinds of drinks and juices. As from the literature survey it is clear that sodium saccharine reported to have some carcinogenic properties when it is used in concentration more than the prescribed amount. The main objective of the study is to detect, determine & quantify the saccharin level in commonly used beverages including carbonated & cola drinks, fruit juices, nectars and energy drinks (non alcoholic) and other commonly used products available in the market.In this study 100 samples of different beverages were analysed. These samples were first degassed for about 30 minutes and then 10ml in 100ml of distilled water was mixed. High Performance liquid chromatographic (HPLC) technique was used for the sample analysis. The analysis was carried out on C18 column in isocratic conditions. The mobile phase contained phosphate buffer of KH2PO4 (0.0125mol/L) and acetonitrile (HPLC grade) in composition of 80:20 ratio (80% Phosphate buffer & 20% Acetonitrile) at pH of 2.5 and ʎmax of 270nm. The results shows that beverages from the renowned companies have shown a very minute and undetectable concentration of Sodium Saccharin. While samples of the beverages especially fruit juices from the locally manufacturing companies shown some higher concentration of sodium saccharin. So this method can be used to accurately determine and quantify sodium saccharine in energy drinks.