Abstract
Background:
The natural products which have convey into being direct medicinal relevance as drug entity many others can function chemical models or templates for the design, synthesis, and semi synthesis of new substances for disease remedy of person.
Objective:
To determine the phytochemicals and test the hepatoprotective activity of Punica granatum in CCl4 triggered liver cells in rabbits with the aid of biochemical evaluation of ALT and AST of plant extract to govern the hepatitis disorder.
Methods:
Rabbits were divided into four groups. Each group consists of six animals (n=6) as follow:
Normal Group: Normal and healthy rabbits.
Control Group: The liver damaged rabbits that received CCl4 + olive oil i.p. for 7 days.
Treatment Group-I: Rabbits that received CCl4 + olive oil for 7 days and then treated with extracts of Punica granatum at amount of 500mg orally for 10 days.
Treatment Group-II: Rabbits received CCl4 the same as in Group-I, but extracts of Punica granatum at amount of 1000mg was given orally for 10 days.
The blood samples were collected and allowed to coagulating for 30 min. at 37 ºC. The clear serums were separated at 3000 rpm for 10 min. and subjected to the estimation of biochemical analyses, asparate amino-transferase (AST) and alanine amino-transferase (ALT).
Results:
The result indicated that the root extract of Punica granatum was useful to induced the liver cells in the rabbits to protect against the harmful diseases like hepatitis as compared to its low concentration dose by Least Significant Difference (LSD) test.
Conclusion:
Punica granatum root extract used in hepato protective activity. There is a beat of global understanding about the significance of many plants to protect the liver damaged by hepato-toxins.