Acute Toxicity Evaluation of Methanolic Leef Extract of Putranjiva roxburghii in Swiss Albino Mice

Abstract

Putranjiva roxburghii is a famous herb that has a potent property of reducing inflammation, treating cancer, struggle against harmful microbes, and safeguard the body against harmful molecules. Thus, the study was aimed to determine the acute toxicity profile of the methanolic leaf extract of P. roxburghii and identify any acute adverse effects. Acute toxicity was evaluated in Swiss albino mice after a single oral administration of 2000 mg/kg body weight, with an observation period of eight days. Biochemical markers (ALT, AST, ALP, creatinine, urea, and uric acid) and hematological indices (RBCs, WBCs, PLT, Hb, hematocrit, MCV) were compared with the control group. A histopathological examination of vital organs, including the liver, kidney, spleen, heart, and lungs, was also performed. Biochemical and hematological parameters did not differ significantly from those of the control mice. Histopathological studies of the vital organs indicated the non-toxic effect of the extract. Our study indicates that the methanolic leaf extract of P. roxburghii demonstrates non-toxic effects and could be explored further for its therapeutic potential in disease management.