ISOLATION AND CHARACTERIZATION OF MICROBIAL COMMUNITIES FROM CICER ARIETINUM L. (CHICKPEAS) ROOT NODULES

Abstract

The Agrobacterium of various leguminous plants show a number of variations, and they were identified and classified as Agrobacterium rhizogenes based on their antibiotic resistance, pathogenicity, microbiology, and presence of plasmid. The aim of present study was to isolation and characterization of microbial communities existing in the root nodules of chickpea plants, with a specific focus on the isolation of Agrobacterium species. The effective success of these objectives delivers appreciated insights into the diversity and potential functional characters of microbial communities associated with root nodules of chickpeas, specifically highlighting the existence of Agrobacterium as a significant component. The isolates from the root nodules were grown on LB agar media. Subsequently, the identification of Agrobacterium was carried out using various tests, including the antibiotic resistance test, gram-staining, microscopy, and pathogenicity test. As well as the plasmid DNA of the isolated bacterial strain was extracted and confirmed by the gel electrophoresis. Once the presence of Agrobacterium will be confirmed, knockouts can be generated by removing the tumor-causing genes from the Ti plasmid (tumor-inducing), resulting in the creation of Agrobacterium mutants. The mutants can be then subjected to sequencing analysis.